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analysis immunofluorescence images  (Olympus)


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    Olympus analysis immunofluorescence images
    Analysis Immunofluorescence Images, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 24440 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laser+confocal+microscope+imaging+analysis/FV3000+Confocal+Laser+Scanning+Microscope/pm41366031-326-3-9
    Average 99 stars, based on 24440 article reviews
    analysis immunofluorescence images - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Alcohol promotes CPT1A-induced lipid metabolism disorder to sentinel-regulate acute pancreatitis
    Article Snippet: The MMP was assessed via a JC-1 probe (Beyotime, Shanghai, China, 2006) according to the manufacturer’s instructions. .. In brief, PECs were stained utilizing a JC-1 probe and later assayed via FACS or Olympus laser confocal microscope imaging analysis. .. FlowJo software utilizes the PE-H (MFI)/FITC-H (MFI) ratio to represent alterations in the MMP for FACS analysis.

    FACS:

    Article Title: Alcohol promotes CPT1A-induced lipid metabolism disorder to sentinel-regulate acute pancreatitis
    Article Snippet: The MMP was assessed via a JC-1 probe (Beyotime, Shanghai, China, 2006) according to the manufacturer’s instructions. .. In brief, PECs were stained utilizing a JC-1 probe and later assayed via FACS or Olympus laser confocal microscope imaging analysis. .. FlowJo software utilizes the PE-H (MFI)/FITC-H (MFI) ratio to represent alterations in the MMP for FACS analysis.

    Microscopy:

    Article Title: Alcohol promotes CPT1A-induced lipid metabolism disorder to sentinel-regulate acute pancreatitis
    Article Snippet: The MMP was assessed via a JC-1 probe (Beyotime, Shanghai, China, 2006) according to the manufacturer’s instructions. .. In brief, PECs were stained utilizing a JC-1 probe and later assayed via FACS or Olympus laser confocal microscope imaging analysis. .. FlowJo software utilizes the PE-H (MFI)/FITC-H (MFI) ratio to represent alterations in the MMP for FACS analysis.

    Imaging:

    Article Title: Alcohol promotes CPT1A-induced lipid metabolism disorder to sentinel-regulate acute pancreatitis
    Article Snippet: The MMP was assessed via a JC-1 probe (Beyotime, Shanghai, China, 2006) according to the manufacturer’s instructions. .. In brief, PECs were stained utilizing a JC-1 probe and later assayed via FACS or Olympus laser confocal microscope imaging analysis. .. FlowJo software utilizes the PE-H (MFI)/FITC-H (MFI) ratio to represent alterations in the MMP for FACS analysis.



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    Image Search Results


    Fig. 5 RPP3 is recruited to autophagosomes under TM-induced ER stress. (A) RPP3 showed the punctate structure under TM-induced ER stress. GFP-RPP3 was expressed in protoplasts before TM treatment as control. The protoplasts then were incubated in tunicamycin (TM, 50 µg ml−1), Concanamycin (ConA, 1 µM), or tunicamycin (TM, 50 µg ml−1) + Concanamycin (ConA, 1 µM) containing media for 24 h. Bar = 10 µm. (B) Quantification of the autophagosomes (APG) per cell. Bars represent the mean (±SD) of at least 30 cells from three biological replicates. (C) RPP3-mCherry puncta colocalized with GFP-ATG8 on autophagosomes. The white arrowheads denote the autophagosomes induced by 50 µg ml−1 TM and/or 1 µM ConA treatment. Bar = 10 µm. The right panel shows the Pearson’s coefficient analysis of the colocalization between RPP3- mCherry and GFP-ATG8 under different treatments. Bars represent the mean (±SD) of at least 30 cells from three biological replicates. (D–E) Autophagic flux analysis of p35S::GFP-ATG8b and p35S::RPP3-GFP by TM treatment. GFP-ATG8b was introduced as the positive control. Each group was incubated in media with or without 50 µg ml−1 TM for 12 h. In addition, the CK group and TM treatment group were supplied with 1 µM ConA.

    Journal: Plant & cell physiology

    Article Title: Identification of New ATG8s-Binding Proteins with Canonical LC3-Interacting Region in Autophagosomes of Barley Callus.

    doi: 10.1093/pcp/pcac015

    Figure Lengend Snippet: Fig. 5 RPP3 is recruited to autophagosomes under TM-induced ER stress. (A) RPP3 showed the punctate structure under TM-induced ER stress. GFP-RPP3 was expressed in protoplasts before TM treatment as control. The protoplasts then were incubated in tunicamycin (TM, 50 µg ml−1), Concanamycin (ConA, 1 µM), or tunicamycin (TM, 50 µg ml−1) + Concanamycin (ConA, 1 µM) containing media for 24 h. Bar = 10 µm. (B) Quantification of the autophagosomes (APG) per cell. Bars represent the mean (±SD) of at least 30 cells from three biological replicates. (C) RPP3-mCherry puncta colocalized with GFP-ATG8 on autophagosomes. The white arrowheads denote the autophagosomes induced by 50 µg ml−1 TM and/or 1 µM ConA treatment. Bar = 10 µm. The right panel shows the Pearson’s coefficient analysis of the colocalization between RPP3- mCherry and GFP-ATG8 under different treatments. Bars represent the mean (±SD) of at least 30 cells from three biological replicates. (D–E) Autophagic flux analysis of p35S::GFP-ATG8b and p35S::RPP3-GFP by TM treatment. GFP-ATG8b was introduced as the positive control. Each group was incubated in media with or without 50 µg ml−1 TM for 12 h. In addition, the CK group and TM treatment group were supplied with 1 µM ConA.

    Article Snippet: Confocal microscopy and colocalization analysis Confocal imaging was performed using a model FV3000 laser scanning confocal microscope (Olympus).

    Techniques: Control, Incubation, Positive Control